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Vitamin B12 plays a vital role in metabolism, nerve function, and the formation of healthy red blood cells

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ROS levels were quantified by observing cells with an inverted fluorescence microscope and measuring the average fluorescence intensity within Myocytes using Image J software after pre-culturing with various concentrations of AX for 6 hours, followed by 2 hours of thermal stress at 37C, with 6 replicates per group

The variance is typically within acceptable experimental error margins